Buchi Rotavapo R-220 SE Manuel d'utilisateur télécharger pdf (Page 48)

5 Putting into operation

48 R-220 SE Operation Manual, Version F

The following concentrations per germ were applied for every contaminant:

germs/ml

5.11.1 Controlled contamination and distillation

5 l of sterile distilled water were ﬁlled into the evaporating ﬂask, contaminant was added to reach the

corresponding concentrations per germ and 1 l was distilled off under the following conditions:

Heating bath set to 60 °C, vacuum set to 42 mbar (corresponds to a boiling point of water of 30 °C),

chiller set to 7 °C.

Afterwards a sample of the distillate was taken (Sample 1 in the table) by means of a sterile pipette

and bottle for determination of germ count.

5.11.2 Disinfection

The evaporating and the receiving ﬂask were emptied and the evaporating ﬂask was rinsed with

water.

1.5 l of isopropanol were ﬁlled into the evaporating ﬂask and the evaporation was started under the

following conditions:

Heating bath set to 60 °C, vacuum set to 250 mbar (corresponds to a boiling point of isopropanol of

50 °C), chiller set to 7 °C.

At ﬁrst, the chiller was switched off until the vapor passed the whole instrument. Then the chiller was

switched on again and 0.75 l were distilled off. Afterwards the evaporting and the receiving ﬂask were

emptied and the instrument was dried at a vacuum of < 20 mbar.

5.11.3 Sampling for determination of the germ count

The evaporating ﬂask was ﬁlled with 2 l of sterile distilled water and 0.75 l were distilled off under the

following conditions:

Heating bath set to 60 °C, vacuum set to 42 mbar (corresponds to a boiling point of water of 30 °C),

chiller set to 7 °C.

A sample of the distillate (Sample 2 in the table) and of the residue within the evaporating ﬂask

(Sample 3 in the table) was taken for the determination of the germ count.

5.11.4 Final cleaning and drying

The evaporating and the receiving ﬂask were emptied and the evaporating ﬂask was rinsed with

water.

1.5 l of isopropanol were ﬁlled into the evaporating ﬂask and the distillation was started under the

following conditions:

Heating bath set to 60 °C, vacuum set to 250 mbar (corresponds to a boiling point of isopropanol of

50 °C), chiller set to 7 °C.

At ﬁrst, the chiller was switched off until the vapor passed the whole instrument. Then the chiller was

switched on again and 0.75 l were distilled off. Afterwards the evaporting and the receiving ﬂask were

emptied and the instrument was dried at a vacuum of < 20 mbar.

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Buchi Rotavapo R-220 SE Manuel d'utilisateur Page 48