Buchi PrepChrom C-700 Manuel d'utilisateur télécharger pdf (Page 81)

BÜCHILabortechnikAG Operation|6

PrepChromC700 81/110

6.5 Equilibrationandinjectionmodes

u Chooseanequilibrationmodetoconditionthecolumnbeforeinjection.

Mode Action

None Nocolumnconditioningbeforeinjec

tion

Equilibration Columnconditioningbeforeinjection;

timedesignation

Equil.+Dryload Columnconditioningbeforeinjection;

notiming;nopossibilityforloopinjec

tionatthe6wayelectricalvalve

u Chooseinjectionmode

Mode Action

Auto Noinjectionthroughtheloopatthe6

wayelectricalvalve;directloadingof

thesampleontopofthecolumn;starts

directlyafterequilibration(ifactivated)

Valveinjection Injectionviatheloopatthe6wayelec

tricalvalve;startsdirectlyafterequili

bration(ifactivated)

Waitforinjection Noinjectionviatheloopatthe6way

electricalvalve;directloadingofthe

sampleontopofthecolumnwhichhas

tobeconfirmedmanually;startsafter

equilibration

Valveinj.+Wait Injectionviatheloopatthe6wayelec

tricalvalvewhichhastobeconfirmed

bytheuser

6.6 Makealiquidinjection

Theloadingandinjectioncanbedonewithoutstoppingthepump.

u Fillasyringewiththevolumeofsamplechosenandplaceitintheinjection

adapterofthe6wayelectricalvalve.

• Keepthesyringeontheadaptertoavoidaleakofthesample.

u Filltheloopwiththecontentofthesyringe.Ifthequantityinjectedis

greaterthanthecapacityoftheloop,thesurplusflowsinthewastebottle.

• Defaultloopvolume:2mL

u Createanewmethodtogetaccesstothesettingsmenu.

• 6.4 Create a new method [} page75]

u SelectthetabSettings.

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Buchi PrepChrom C-700 Manuel d'utilisateur Page 81