Buchi ELS Detector C-650 Manuel d'utilisateur télécharger pdf (Page 37)

C-650 Operation Manual, Version B

5 Putting into operation

5.9.5 Column noise test

NOTE

When strongly retained compounds are slowly eluted from the column, excessive noise will be

observed.

To determine the column noise:

Turn off the pump and connect the column.

Restart the pump and allow the mobile phase to ﬂow through the system. It is suggested that

you ﬂush the column with a strong solvent for a few minutes before attaching it to the detector.

Set the gain to 12 and monitor the baseline for a few minutes. A suitable column will provide a

baseline that is 20–50mV above the solvent baseline.

NOTE

If the mobile phase contains acidic modiﬁers (e.g. CF

COOH), disconnect the detector and wash

the Chromatography system for 12h before starting to analyze unknown samples. This wash

should be performed after the column noise test is completed, but need not to be performed

after each analysis.

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Buchi ELS Detector C-650 Manuel d'utilisateur Page 37